Enzyme Carrier Resins - Prototypes

Redstone Separations AB develops and produces novel types of enzyme carriers (EC) resins for immobilization of biological catalysts such as enzymes. 

Our novel resin architecture technology allows for new material morphologies and material chemistry/properties with improved enzyme immobilization and facilitated substrate / product diffusion. 

EC Resins are macroporous resins with large pores that can harbour a large variety of enzymes and that allow easy access and release of starting compounds and products of the catalysis.

Typically EC resins have the following properties:

  • Polymeric backbone
  • Various chemical properties of the backbone can be adapted
  • Incorporation of chemical functionality for covalently anchoring enzymes: Examples are amino or epoxy groups
  • Pore size ranges: approx 500 Å, 1000 Å or 2000 Å
  • Particle size: 40-75 µm or 75-200 µm or even larger ones for better sedimentation

 

Preliminary first results of novel hydrophilic carrier

A highly polar and hydrophilic prototype resin is in development with promising performance in aqueous enzyme supported conversions.

A highly hydrophobic substrate was first tested for non-specific binding to the concept material. Restone EC prototype had over 90 % free available substrate whereas other conventional Enzyme carriers on the market bound a large portion of the substrate non-specifically to their resin backbone leaving only 40 % of valuable substrate accessible for enzyme conversion to product.

Redstone concept enzyme carrier displayed a high enzyme immobilization efficiency of over 85 %. The immobilized enzyme construct is very stable over at least 3 test rounds with no reduction in enzyme activity. Below is the picture of the wet resins with a particle size around 100 – 300 µm in swollen state. 

A broad variety of EC materials can be designed and provided, also at large industrial scale.

Pls see our custom resin development service.

Pls inquire: Email: info@redstone-sep.com 

Phone: +46-738-156-130  (Call times during CET: 8.00 – 18.00)