Custom made tailored resins
Design of resin morphology
- Particle size
- Pore size
- Rigidity / cross-linking degree
Temiz examples resin, microscopic picture.
Examples of average particle sizes that can be delivered:
- sub 5 µm particles for eg UPLC or assays
- 5 or 10 µm for HPLC
- 50 µm for low pressure LC
- 100 um for solid phase synthesis
- 500 µm for industrial purifications
SEM example of large pores. Magnification 50.000 x
Examples of pore sizes that can be engineered:
- 100 Å (or below) for small molecules
- 300 – 500 Å for peptides and oligomers
- 1000 Å for proteins, antibodies, etc
- >>1000 Å for virus, cells, complex biologics
Resin selectivity
- General resin polarity design
- Construction of multi-functional groups
- Formation of binding cavities with coordinated functional groups
- Anchoring group for immobilization of a ligand eg protein or small molecule ligand
Selectivity principles are based on complementary multi-functional affinity surfaces and cooperative binding, either as multi-functional binding surfaces or as advanced constructed binding site cavities, to exihibit heightened affinity and selecitivity for the desired target compound.
Multi-functional surface
Multi-functional resins display 2 or more distinct chemical surface functionalities. Those exhibit surface binding interactions with the desired target molecules. Multi-functional surfaces offer increased selectivity and/or affinity performance towards target molecules.
The chemical groups can be designed and synthesized in discussion with the customer.
Constructed cavity site
Constructed cavity resins contain accessible binding sites that entail synergistic functional groups that cooperate to bind a target molecule. Cooperative interactions are expected to exhibit a higher selectivity and/or affinity performance towards target molecules.
The chemical groups and their positions can be designed and synthesized in discussion with the customer.
Anchoring groups
A range of covalent anchoring groups can be provided for covalent immobilization of a variety of biological protein ligands (eg Prot A) and other small molecule ligands:
- Primary amino groups via glutaraldehye
- Maleimide groups for SH-moeities
- Epoxy groups for amino groups
- Carboxylic acid groups – via acid activation
- Different Linkers can be incorporated
- Other surface chemistries
Examples of compounds to be separated or removed
Difficult separations are for example the removal of toxic or unwanted contaminants from natural biological extracts or synthetic mixtures, or biotechnological process solutions. Natural unwanted compounds can be all types of positively, neutral or negatively charged compounds. An example of unwanted non-natural compounds are agricultural or industrial residues such as pesticides or other chemicals.
Retrieving target compounds from very difficult mixtures is a challenge for many areas in biotherapeutics. Examples are biopharmaceutical or fermentation broths, cell culture solutions, or plant extracts. Separation of proteins, antibodies, oligonucleotides and other biological large molecules are among the most demanding targets. Larger biological targets such as mRNA vaccines, virus or cells require the largest porosity.
Steps of tailor-made resin development
Depending on the separation challenge, first in-house “one-functionality” resins can be used, or more advanced multi-functional resins or highly engineered constructed cavity resins can be synthesized and evaluated.
A typical resin development project for a purification problem will go through the standard project phases with defined goals and gates to go to the next phase.
For project start, customer may send samples of material to be purified to Redstone Separations AB in Sweden.
- In-house library screening for first resin hits / or de-novo synthesis of new resin designs
- Optimization of hit resins for further performance
- Optimization of resin synthesis at lab scale
- Scale-up to pilot scale
- Scale-up of optimized resin to commercial / industrial scale
Redstone can deliver tailor made resin to customer and supports resin testing or implementation after each stage.
Redstone is an expert resin producer and can provide resins at g, kg and at ton-scale.
Project planning & conduction
Please contact us for further discussion as this is a case-by-case evaluation.
We will present a project plan and a quote.
Email: info@redstone-sep.com
Phone: +46-738-156-130 (Call times during CET: 8.00 – 18.00)